Antimicrobial sensitivity of Listeria monocytogenes isolated from beef
Resumo
The objective of this study was to investigate the antimicrobial susceptibility of Listeria monocytogenes previously isolated from beef. The experiment used ground beef, commercialized in the city of Uberaba, Minas Gerais, Brazil. Using the Kirby-Bauer method, analyzes of antimicrobial susceptibility were carried out in the 14 isolates of Listeria monocytogenes. All strains (100%) showed sensitivity to antimicrobials: amoxicillin, ampicillin, gentamicin, penicillin, teicoplanin and vancomycin; Six strains (42.86%) presented sensitivity to sulfamethoxazole / trimethoprim and tetracycline; 3 strains (21.43%) presented sensitivity to erythromycin and 2 strains (14.29%) showed sensitivity to ciprofloxacin. While 8 strains (57.14%) presented intermediate resistance to ciprofloxacin and tetracycline. All strains isolated showed resistance to cefazolin, cefepime, cefotaxime, clindamycin, oxacillin and polymyxin b. The antimicrobial resistance of Listeria monocytogenes poses serious risks to public health, due to its seriousness in affecting vulnerable segments of the human population, due to the wide presence of this bacterium in the environment, being isolated from food processing environments, water effluents to industries and causing possible contamination of various foodstuffs.
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Referências
BHAT, S.A.; WILLAYAT, M.M.; ROY, S.S.; BHAT, M.A.; SHAH, S.N.; AHMEID, A.; MAQBOOL, S.; GANAYI, B.A. Isolation, molecular detection and antibiogram of Listeria monocytogenes from human clinical cases and fish of Kashmir, India. Comparative Clinical Pathology, v. 22, n. 4, p. 661-665, 2013.
CHEN, B.Y; PYLA, R.; KIM, T.J.; SILVA, J.L.; JUNG, Y.-S. Antibiotic resistance in Listeria species isolated from catfish fillets and processing environment. Letters in applied microbiology, v. 50, n. 6, p. 626-632, 2010.
CLSI, Clinical and Laboratory Standards Institute. Methods for Antimicrobial Dilutions and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria (M45-A3), 3.ed. CLSI guideline M45. Wayne, PA: Clinical and Laboratory Standards Institute, 2015.
CLSI, Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing (M100-S26). 26. ed. CLSI supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute, 2016.
CLSI, Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing (M100-S22). 22. ed. CLSI supplement M100S. Wayne, PA: Clinical and Laboratory Standards Institute, 2012.
COMITÉ DE L'ANTIBIOGRAMME DE LA SOCIÉTÉ FRANÇAISE DE MICROBIOLOGIE. Recomendations 2016: Version 1.0 de Février, France 2016. Available in: . Acesso em: 01/01/2017.
CONTER, M.; PALUDI, D.; ZANARDI, E.; GHIDINI, S.; VERGARA, A.; IANIERI, A. Characterization of antimicrobial resistance of foodborne Listeria monocytogenes.
International Journal of Food Microbiology, v.128, n.3, p.497-500, 2009.
ENNAJI, H.; TIMMINOINI, M.; ENNAJI, M.M.; COHEN, N. Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco. Infection and Drug Resistance, v. 1, p. 45-50, 2008.
EUCAST, European Committee on Antimicrobial Susceptibility Testing et al. Breakpoint tables for interpretation of MICs and zone diameters: Version 7.0, valid from 2017-01-01, 2017. Available in:< http://www.eucast.org>. Accessed in: 01/01/2017.
FAO & WHO, Food and Agriculture Organization and World Health Organization. Risk Assessment of Listeria monocytogenes in Ready-to-Eat Foods, Microbiological Risk Assessment Series 4, Interpretive Summary, 2004. Available in: . Accessed in: 27 jul. 2016. ISBN 978-92-5-105762-9
FDA, Food and Drug Administration. U.S. Department of Health and Human Services. Center for Food Safety and Applied Nutrition. Draft Guidance for Industry: Control of Listeria monocytogenes in Ready-To-Eat Foods, 2017. Available in: . Accessed in: 13/02/2017.
FILGUEIRAS, C.T.; VANETTI, M.C.D. Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes. Brazilian Archives of Biology and Technology, Curitiba, v. 49, n. 3, p. 405-409, 2006.
FORSYTHE, STEPHEN J. Microbiologia da segurança dos alimentos. 2.ed. Porto Alegre: Artmed, 2013. 620p.
FRANCO, B.D.G.M.; LANDGRAF, M. Microbiologia dos Alimentos. São Paulo: Editora Atheneu, 2008. p.182.
HERNÁNDEZ, BALTASAR BALSALOBRE; HERNÁNDEZ-GODOY, JOAQUÍN. Resistencias a antibióticos en Listeria monocytogenes y Salmonella enterica aislados de alimentos de origen animal. Revista de Salud Ambiental, v. 4, n. 1-2, p. 42-46, 2004.
JAY, J. M. Listerioses de origem animal. In: ______. Microbiologia de alimentos. 6. ed. Porto Alegre: Artmed, 2005. cap. 25, p. 517-542, 711 p.
KHEN, B.K., LYNCH, O.A., CARROLL, J., MCDOWELL, D. A. and DUFFY, G. (2015), Occurrence, Antibiotic Resistance and Molecular Characterization of Listeria monocytogenes in the Beef Chain in the Republic of Ireland. Zoonoses Public Health, 62: 11–17. doi:10.1111/zph.12106
LECUIT, M. Human Listeriosis and Animal Models. Microbes and Infeccion. v.9, p.1216-1225, 2007.
MANTILLA, S.P.S.; FRANCO, R.M.; OLIVEIRA, L.A.T.; SANTOS, E.B.; GOUVÊA, R. Ocorrência de Listeria spp. em amostras de carne bovina moída comercializadas no município de Niterói, RJ, Brasil. Ciência e Agrotecnologia, Lavras, v.31, n.4, p.1225-1230, 2007.
MANTILLA, S.P.S.; FRANCO, R.M.; OLIVEIRA, L.A.T.; SANTOS, E.B.; GOUVÊA, R. Resistência antimicrobiana de bactérias do gênero Listeria spp: isoladas de carne moída bovina. Brazilian Journal of Veterinary Research and Animal Science, v. 45, n. 2, p. 116-121, 2008.
PALMA, J.M.; LISBOA, R.C.; RODRIGUES, D.P.; SANTOS, A.F.M.; HOFER, E.; SANTANA, A.P. Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil. Pesquisa Veterinária Brasileira, v. 36, n. 10, p. 957-964, 2016.
SILVA, N.; JUNQUEIRA, V.C.A.; SILVEIRA, N.F.A.; TANIWAKI, N.H.; GOMES, R.A.R.; OKASAKI, M.M. Manual de Métodos de Análise Microbiológica de Alimentos e Água. 5. ed. São Paulo: Livraria Blucher, 2017. 560p.
TORTORA, G.J.; CASE, C.L.; FUNKE, B.R. Microbiologia. 12. ed. Porto Alegre: Artmed Editora, 2016. 964p.
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