Effect of substituion of different levels of nano dicalcium phosphate (CaHPO4) with dicalcium phosphate on serum blood biochemical parameters, intestinal morphology and microflora and bone characteristics in broiler Japanese quails
Resumo
The aim of the current study was to determine the effect of substituion of different levels of nano dicalcium phosphate (NDCP) with dicalcium phosphate (DCP) in quails dietary regims on serum blood biochemical parameters, intestinal morphology and microflora and bone characteristics in broiler Japanese quails. A total of 360 quails were used in experimental randomized design from with 6 treatments of DCP and NDCP (60, 80 and 100 percetnage) and five replicates with 12 quails perv each replicate. The experimental period lasted 35 days. At the end of the study period blood samples were taken to determine serum blood bichemical and liver enzemye activities. For intestinal morphology determination the sample tissue of midway between jejunum and ileum were collected and samples of digesta from ileum were taken for microbial contents assessment. Bone characteristics such as tibia ash, phosphorus, calcium, and the ratio of tibia weight to length left tibia were analyzed. Results showed significant differences in serum Alanine transaminase (ALT), Aspartate transaminase (AST), and phosphorus levels among the study groups (p≤0.05). Additionally, there were significant differences in tibia ash content, phosphorus and calcium levels, weight to length ratio, and tibia weight (p≤ 0.05). The lowest villus height and width ratio was observed in the 60% dicalcium phosphate treatment, and the lowest crypt depth was found in the 100% dicalcium phosphate treatment. Data also showed that there were significant effect of dietray NDCP for intestinal microflora in experimental quails (p≤0.05). Overall, we could conclude that replacing standard dicalcium phosphate with nano dicalcium phosphate did not negatively impact the health of the quails and safely enhanced bone growth through nano mineral supplementation.
Palavras-chave
Referências
ABDELFATAH, R., MOHAMED, I., et al. (2023). Impact of dietary dicalcium phosphate nanoparticles on productive performance of broiler chickens. Aswan University Journal of Environmental Studies, 4(5), 419–429.
AL-BEITAWI, N. A., MOMANI, S. M., EL-SHURAYDEH, K. N., & BLÁHA, J. (2017). Effect of nanoclay minerals on growth performance, internal organs and blood biochemistry of broiler chickens compared to vaccines and antibiotics. Journal of Applied Animal Research, 45(1), 543–549.
AL-BEITAWI, N., ELSHURAYDEH, K., AL-FAQEIH, M., ZYADEH, M. (2017). Effect of an aqueous nanosuspension of clay minerals on the performance, carcass characteristics, and internal organs of broilers. Journal of Nanotechnology in Nanomedicine and Nanobiotechnology, 4, 13.
AOAC.Official methods of analysis, Association of official analytical chemists. AOAC Press, Gaithersburg, USA.2005.
BORDA-MOLINA, D., ROTH, C., HERNÁNDEZ-ARRIAGA, A., RISSI, D., VOLLMAR, S., RODEHUTSCORD, M., et al. (2020). Effects on the ileal microbiota of phosphorus and calcium utilization, bird performance, and gender in Japanese quail. Animals, 10(5), 885.
BROOKS, M. A., GRIMES, J. L., LLOYD, K. E., VERISSIMO, S., SPEARS, J. W. (2013). Bioavailability in chicks of zinc from zinc propionate. Journal of Applied Poultry Research, 22(2), 153–159.
CHEN, X., ZHANG, Q., APPLEGATE, T. J. (2016). Impact of dietary branched chain amino acids concentration on broiler chicks during aflatoxicosis. Poultry Science, 95(6), 1281–1289.
CIURESCU, G., VASILACHI, A., & GROSU, H. (2020). Efficacy of microbial phytase on growth performance, carcass traits, bone mineralization, and blood biochemistry parameters in broiler turkeys fed raw chickpea (Cicer arietinum L., cv. Burnas) diets. Journal of Applied Poultry Research, 29(1), 171–184.
DAVID, L. S., ABDOLLAHI, M. R., BEDFORD, M. R., RAVINDRAN, V. (2021). True ileal calcium digestibility in soybean meal and canola meal, and true ileal phosphorus digestibility in maize-soybean meal and maize-canola meal diets, with and without microbial phytase, for broiler growers and finishers. British Poultry Science, 62(2), 293–303.
DE SOUSA, J. P. L., ALBINO, L. F. T., VAZ, R., RODRIGUES, K. F., DA SILVA, G. F., RENNO, L. N., et al. (2015). The effect of dietary phytase on broiler performance and digestive, bone, and blood biochemistry characteristics. Brazilian Journal of Poultry Science, 17, 69–76.
EL-SHEIKH, T. M., ABOU-ELNAGA, M. K. (2018). Effect of nano-dicalcium phosphate supplementation on eggshell structure and calcium and phosphorus utilization. Egyptian Journal of Nutrition and Feeds, 21(3), 833–846.
GROSS, K. A., ANDERSONS, J., MISEVICIUS, M., SVIRKSTS, J. (2014). Traversing phase fields towards nanosized beta tricalcium phosphate. Key Engineering Materials, 587, 97–100.
LABHASETWAR, V. (2005). Nanotechnology for drug and gene therapy: The importance of understanding molecular mechanisms of delivery. Current Opinion in Biotechnology, 16(6), 674–680.
MAKOLA, M. D., et al. (2021). Dietary nano-dicalcium phosphate improves immune response and intestinal morphology of broiler chickens. South African Journal of Animal Science, 51(3), 362–370.
MATUSZEWSKI, A., ŁUKASIEWICZ, M., NIEMIEC, J. (2020). Calcium and phosphorus and their nanoparticle forms in poultry nutrition. World's Poultry Science Journal, 76(2), 328–345.
MANN, K., MANN, M. (2015). Proteomic analysis of quail calcified eggshell matrix: A comparison to chicken and turkey eggshell proteomes. Proteome Science, 13, 1–19.
MARCHIORI, M. S., OLIVEIRA, R. C., SOUZA, C. F., BALDISSERA, M. D., RIBEIRO, Q. M., WAGNER, R., et al. (2020). Curcumin in the diet of quail in cold stress improves performance and egg quality. Animal Feed Science and Technology, 254, 114192.
METSON, G. S., BENNETT, E. M., ELSER, J. J. (2012). The role of diet in phosphorus demand. Environmental Research Letters, 7(4), 44043.
NOURMOHAMMADI, R., HOSSEINI, S. M., FARHANGFAR, H. (2011). Effect of citric acid and microbial phytase on serum enzyme activities and plasma minerals retention in broiler chicks. African Journal of Biotechnology, 10(62), 13640–13650.
NRC.National Research Council Nutrient requirements of poultry.9thRevEd., Washington, DC. National Academy Press.1994.
NUAMAH, E., et al. (2024). Unlocking phytate with phytase: A meta-analytic view of meat-type chicken muscle growth and bone mineralization potential. Animals, 14(14), 2090.
OGNIK, K., STĘPNIOWSKA, A., KOZŁOWSKI, K. (2017). The effect of administration of silver nanoparticles to broiler chickens on estimated intestinal absorption of iron, calcium, and potassium. Livestock Science, 200, 40–45.
POINERN, G. E., BRUNDAVANAM, R. K., MONDINOS, N., JIANG, Z. T. (2009). Synthesis and characterization of nanohydroxyapatite using an ultrasound-assisted method. Ultrasonics Sonochemistry, 16, 469–474.
SOUSA, J. P. L., et al. (2015). The effect of dietary phytase on broiler performance and digestive, bone, and blood biochemistry characteristics. Revista Brasileira de Ciência Avícola, 17(1), 69–76.
STANQUEVIS, C. E., FURLAN, A. C., MARCATO, S. M., DE OLIVEIRA-BRUXEL, T. M., PERINE, T. P., FINCO, E. M., et al. (2021). Calcium and available phosphorus requirements of Japanese quails in early egg-laying stage. Poultry Science, 100(1), 147–158.
SWAIN PS, RAJENDRAN D, RAO SB, DOMINIC G. (2015). Preparation and effects of nano mineral particle feeding in livestock.A. review. Vet. World,8(7):888-891.
VIJAYAKUMAR, M. P., BALAKRISHNAN, V. (2015). Assessment of calcium phosphate nanoparticles as a safe mineral supplement for broiler chicken. Indian Journal of Science and Technology, 8(7), 608–613.
VIJAYAKUMAR, M. P., BALAKRISHNAN, V. (2014). Evaluating the bioavailability of calcium phosphate nanoparticles as a mineral supplement in broiler chickens. Indian Journal of Science and Technology, 7(10), 1475–1480.
WEI, X., HUANG, X. (2019). Origin, taxonomy, and phylogenetics of rice. In Rice (pp. 1–29). Elsevier.
Apontamentos
- Não há apontamentos.
Visitas