Ferramentas para a avaliação de células e tecidos somáticos após a criopreservação em mamíferos. Uma Revisão

Cibelle Anne dos Santos Costa, Alana Azevedo Borges, Maria Valéria de Oliveira Santos, Luiza Bento de Queiroz Neta, Alexsandra Fernandes Pereira

Resumo


A conservação de células e tecidos somáticos possui um papel importante para a preservação da biodiversidade, otimização em manejos reprodutivos e sistemas terapêuticos. Isso é possível através do aperfeiçoamento de técnicas de criopreservação e uso desse material genético em outras biotecnologias reprodutivas, como a transferência nuclear (clonagem). Nesse contexto, faz-se necessário uma análise precisa da amostragem biológica após a criopreservação, avaliando a viabilidade e, consequentemente, a eficiência da técnica de criopreservação empregada. Assim, essa revisão objetiva abordar as várias estratégias empregadas na determinação da eficiência dos métodos de criopreservação de células e tecidos somáticos, enfatizando seus aspectos teóricos e práticos em algumas espécies de mamíferos.


Palavras-chave


conservação; cultivo in vitro; análise histológica.

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