Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis

Flaviane Alves de Pinho, Geórgia Brenda Barros Alves, Maria do Socorro Pires e Cruz

Resumo


Leishmaniose visceral canina (LVC) é uma zoonose causada por Leishmania (Leishmania) infantum chagasi no continente Americano. Poucos estudos demonstraram as características imunopatológicas em linfonodo poplíteo e fígado de cães infectados em área endêmica de Teresina, Piauí, Brasil. Assim, nós avaliamos as alterações histopatológicas e a presença de células T CD4+ e CD8+ em linfonodo poplíteo e fígado de nove cães com manifestações clínicas (G1) e sete cães sem manifestações clínicas (G2) naturalmente infectados com L. (L.) infantum chagasi. Fragmentos de linfonodo poplíteo e fígado foram obtidos para análise histopatológica e imunoistoquímica. As principais manifestações clínicas foram linfadenopatia (80%) e lesões de pele (70%). Na análise histopatológica do linfonodo foi observada uma depleção de células da região cortical. Não havia diferença significante entre os grupos entre células T CD4+ e CD8+ mas havia uma correlação positiva entre essas células em  G1 e G2. No fígado, o infiltrado inflamatório era focal consistindo de células mononucleares nas regiões periportal e intralobular, em ambos os grupos. O infiltrado inflamatório era positivo na imunomarcação para células T CD4e CD8, mas sem diferença significante entre os grupos. Entretanto, havia uma correlação positiva entre as células T CD4+ e CD8+ no G1. Na análise da imunomarcação também  mostrou  mais amastigotas e antígenos de Leishmania no fígado e linfonodo poplíteo em G1 em relação ao G2. O papel das células T CD4+ e CD8+ parece não estar relacionada ao estado clínico dos cães com LVC.


Palavras-chave


Leishmania,;canino; CD4, CD8.

Referências


ABBAS, A.K.; LICHTMAN, A.H.; PILLAI, S. Cellular and molecular immunobiology. 7th ed. Rio de Janeiro: Elsevier, 2011. 592p.

ABRANCHES, P.; SANTO-GOMES, G.; RACHAMIM, N.; CAMPINO, L.; SCHUNUR, L.F.; JAFFE, C.L. An experimental model for canine visceral leishmaniasis. Parasite Immunoly, v. 13, n. 5, p. 537-50, 1991.

ABREU-SILVA, A.L.; CALABRESE, K.S.; MORTARA, R.A.; TEDESCO, R.C.; CARDOSO, F.O.; CARVALHO, L.O.; GONÇALVES DA COSTA, S. C. Extracellular matrix alterations in experimental murine Leishmania (L.) amazonensis infection. Parasitology, v. 128, n. 4, p. 385-90, 2004.

ALEXANDRE-PIRES, G.; DE BRITO, M.T.; ALGUERÓ, C.; MARTINS, C.; RODRIGUES, O.R.; DA FONSECA, I.P.; SANTOS-GOMES, G. Canine leishmaniasis. Immunophenotypic profile of leukocytes in different compartments of symptomatic, asymptomatic and treated dogs. Veterinary Immunology and Immunopathology, v. 137, n. 3-4, p. 275-83, 2010.

ALVES, C.F.; DE AMORIM, I.F.; MOURA, E.P.; RIBEIRO, R.R.; ALVES, C.F.; MICHALICK, M.S.; KALAPOTHAKIS, E.; BRUNA-ROMERO, O.; TAFURI, W.L.; TEIXEIRA, M.M.; MELO, M.N. Expression of IFN-gamma, TNF-alpha, IL-10 and TGF-beta in lymph nodes associates with parasite load and clinical form of disease in dogs naturally infected with Leishmania (Leishmania) chagasi. Veterinary Immunology and Immunopatholy, v. 128, n. 4, p. 349-58, 2009.

BANETH, G.; KOUTINAS, A.F.; SOLANO-GALLEGO, L.; BOURDEAU, P.; FERRER, L. Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part one. Trends in Parasitology, v. 24, n. 7, p. 324-30, 2008.

BEATTIE, L.; PELTAN, A.; MAROOF, A.; KIRBY, A.; BROWN, N.; COLES, M.; SMITH, D.F.; KAYE, P.M. Dynamic imaging of experimental Leishmania donovani-induced hepatic granulomas detects Kupffer cell-restricted antigen presentation to antigen-specific CD8 T cells. PLoS Pathogens, v. 6, n. 3, p. e1000805, 2010.

BRACHELENTE, C.; MÜLLER, N.; DOHERR, M.G.; SATTLER, U.; WELLE, M. Cutaneous leishmaniasis in naturally infected dogs is associated with a T helper-2-based immune response. Veterinary Patholology, v. 42, n. 2, p. 166-75, 2005.

CABRAL, M.; O'GRADY, J.E.; GOMES, S.; SOUSA, J.C.; THOMPSON, H.; ALEXANDER, J. The immunology of canine leishmaniosis: strong evidence for a developing disease spectrum from asymptomatic dogs. Veterinary Parasitology, v. 76, n. 3, p. 173-80, 1998.

CIARAMELLA, P.; OLIVA, G.; LUNA, R.D.; GRADONI, L.; AMBROSIO, R.; CORTESE, L.; SCALONE, A.; PERSECHINO, A. A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. Veterinary Record, v. 141, n. 21, p. 539-43, 1997.

COSTA, F.A.; PRIANTI, M.G.; SILVA, T.C.; SILVA, S.M.; GUERRA, J.L.; GOTO, H. T cells, adhesion molecules and modulation of apoptosis in visceral leishmaniasis glomerulonephritis. BMC Infectious Disease, v. 10, p. 112, 2010.

COSTA, M.M.; LIMA, W.G.; FIGUEIREDO, M.M.; MICHALICK, M.S.; TAFURI, W.L.; TAFURI, W.L. Cervical, mandibular, and parotid lymph nodes of dogs naturally infected with Leishmania infantum: a histopathologic and immunohistochemistry study and its correlation with facial skin lesions. Veterinary Patholology, v. 45, n. 5, p. 613-6, 2008.

CROCKER, P.R.; DAVIES, E.V.; BLACKWELL, J.M. Variable expression of the murine natural resistance gene Lsh in different macrophage populations infected in vitro with Leishmania donovani. Parasite Immunology, v. 9, n. 6, p. 705-19, 1987.

CUNHA, S.; FREIRE, M.; EULALIO, C.; CRITOSVAO, J.; NETTO, E.; JOHNSON, W.D. J.R.; REED, S.G.; BADARO, R. Visceral leishmaniasis in a new ecological niche near a major metropolitan area of Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 89, n. 2, p. 155-8, 1995.

DANTAS-TORRES, F. The role of dogs as reservoirs of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Veterinary Parasitology, v. 149, n. 3-4, p. 139-46, 2007.

FAROOQ, U.; CHOUDHARY, S.; CHACON, A.H.; LEBRUN, E.; SHIMAN, M.I.; HERNANDEZ, J.; MILIKOWSKI, C.; KERDEL, F.A.; ROMANELLI, P. Post-kala-azar dermal leishmaniasis in HIV-infected patients with AIDS: a report of two cases diagnosed in the USA. International journal of dermatology, v. 52, n. 9, p. 1098-104, 2013.

GIUNCHETTI, R.C.; MARTINS-FILHO, O.A.; CARNEIRO, C.M.; MAYRINK, W.; MARQUES, M.J.; TAFURI, W.L.; CORRÊA-OLIVEIRA, R.; REIS, A.B. Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 121, n. 1-2, p. 23-33, 2008.

GUARGA, J.L.; LUCIENTES, J.; PERIBÁÑEZ, M.A.; MOLINA, R.; GRACIA, M.J.; CASTILLO, J.A. Experimental infection of Phlebotomus perniciosus and determination of the natural infection rates of Leishmania infantum in dogs. Acta Tropica, v. 77, n. 2, p. 203-7, 2000.

GUERRA, L.L.; TEIXEIRA-CARVALHO, A.; GIUNCHETTI, R. C.; MARTINS-FILHO, O.A.; REIS, A.B.; CORRÊA-OLIVEIRA, R. Evaluation of the influence of tissue parasite density on hematological and phenotypic cellular parameters of circulating leukocytes and splenocytes during ongoing canine visceral leishmaniasis. Parasitology Research, v. 104, n. 3, p. 611-22, 2009.

HEINZEL, F.P.; SADICK, M.D.; HOLADAY, B.J.; COFFMAN, R.L.; LOCKSLEY, R.M. Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for expansion of distinct helper T cell subsets. TheJournal of Experimental Medicine, v. 169, n. 1, p. 59-72, 1989.

KAZAZI, F.; MATHIJS, J.M.; FOLEY, P.; CUNNINGHAM, A.L. Variations in CD4 expression by human monocytes and macrophages and their relationships to infection with the human immunodeficiency virus. The Journal of general virology, v. 70, p. 2661-72, 1989.

KEENAN, C.M.; HENDRICKS, L. D.; LIGHTNER, L.; WEBSTER, H. K.; JOHNSON, A.J. Visceral leishmaniasis in the German shepherd dog. I. Infection, clinical disease, and clinical pathology. Veterinary Pathology, v. 21, n. 1, p. 74-9, 1984.

LEMOS DE SOUZA, V.; ASCENÇÃO SOUZA, J.; CORREIA SILVA, T. M.; SAMPAIO TAVARES VERAS, P.; RODRIGUES DE-FREITAS, L.A. Different Leishmania species determine distinct profiles of immune and histopathological responses in CBA mice. Microbes and infection / Institut Pasteur, v. 2, n. 15, p. 1807-15, 2000.

MAIA, C.; CAMPINO, L. Cytokine and Phenotypic Cell Profiles of Leishmania infantum Infection in the Dog. Journal of Tropical Medicine, v. 2012, p. 541571, 2012.

MAURÍCIO, I.L.; STOTHARD, J.R.; MILES, M.A. The strange case of Leishmania chagasi. Parasitology Today, v. 16, n. 5, p. 188-9, 2000.

MCELRATH, M.J.; MURRAY, H.W.; COHN, Z.A. The dynamics of granuloma formation in experimental visceral leishmaniasis. Journal of Experimental Medicine, v. 167, n. 6, p. 1927-37, 1988.

MENDONCA, S.C.F.; LUCA, P.M.; MAYRINK, W.; RESTOM, T.G.; CONCEIÇÃO-SILVA, F.; DA-CRUZ, A.M.; BERTHO, A.L.; DA COSTA, C.A.; GENARO, O.; TOLEDO, A.P.C.P.; COUTINHO, S.G. Characterization of human T lymphocyte-mediated immune responses induced by a vaccine against American tegumentary leishmaniasis. The American journal of tropical medicine and hygiene, v. 53, n. 2, p. 195-201, 1995.

MICHALSKY, E.M.; ROCHA, M.F.; DA ROCHA LIMA, A.C.; FRANÇA-SILVA, J.C.; PIRES, M.Q.; OLIVEIRA, F. S.; PACHECO, R.S.; DOS SANTOS, S. L.; BARATA, R.A.;ROMANHA, A. J.; FORTES-DIAS, C.L.; DIAS, E.S. Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies. Veterinary Parasitology, v. 147, n. 1-2, p. 67-76, 2007.

MIRANDA, S.; MARTORELL, S.; COSTA, M.; FERRER, L.; RAMIS, A. Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol. Veterinary Parasitology, v. 144, n. 3-4, p. 251-60, 2007.

MORENO, J.; ALVAR, J. Canine leishmaniasis: epidemiological risk and the experimental model. Trends Parasitology, v. 18, n. 9, p. 399-405, 2002.

MORENO, J.; NIETO, J.; CHAMIZO, C.; GONZÁLEZ, F.; BLANCO, F.; BARKER, D.C.; ALVAR, J. The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy. Veterinary Immunology and Immunopathology, v. 71, n. 3-4, p. 181-95, 1999.

MURRAY, H.W. Tissue granuloma structure-function in experimental visceral leishmaniasis. International journal of experimental pathology, v. 82, n. 5, p. 249-67, 2001.

MÜLLER, I.; KROPF, P.; LOUIS, J. A.; MILON, G. Expansion of gamma interferon-producing CD8+ T cells following secondary infection of mice immune to Leishmania major. Infection and Immunity, v. 62, n. 6, p. 2575-81, 1994.

NYLÉN, S.; MAURYA, R.; EIDSMO, L.; MANANDHAR, K.D.; SUNDAR, S.; SACKS, D. Splenic accumulation of IL-10 mRNA in T cells distinct from CD4+CD25+ (Foxp3) regulatory T cells in human visceral leishmaniasis. The Journal of experimental medicine, v. 204, n. 4, p. 805-17, 2007.

PINELLI, E.; KILLICK-KENDRICK, R.; WAGENAAR, J.; BERNADINA, W.; DEL REAL, G.; RUITENBERG, J. Cellular and humoral immune responses in dogs experimentally and naturally infected with Leishmania infantum. Infection and Immunity, v. 62, n. 1, p. 229-35, 1994.

PINELLI, E.; RUTTEN, V. P.; BRUYSTERS, M.; MOORE, P.F.; RUITENBERG, E. J. Compensation for decreased expression of B7 molecules on Leishmania infantum-infected canine macrophages results in restoration of parasite-specific T-cell proliferation and gamma interferon production. Infection and Immunity, v. 67, n. 1, p. 237-43, 1999.

RALLIS, T.; DAY, M. J.; SARIDOMICHELAKIS, M. N.; ADAMAMA-MORAITOU, K.K.; PAPAZOGLOU, L.; FYTIANOU, A.; KOUTINAS, A.F. Chronic hepatitis associated with canine leishmaniosis (Leishmania infantum): a clinicopathological study of 26 cases. Journal of comparative pathology, v. 132, n. 2-3, p. 145-52, 2005.

REIS, A.B.; MARTINS-FILHO, O. A.; TEIXEIRA-CARVALHO, A.;CARVALHO, M.G.; MAYRINK, W.; FRANÇA-SILVA, J.C.; GIUNCHETTI, R.C.; GENARO, O.; CORRÊA-OLIVEIRA, R. Parasite density and impaired biochemical/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis. Research in veterinary science, v. 81, n. 1, p. 68-75, 2006.

REIS, A.B.; MARTINS-FILHO, O.A.; TEIXEIRA-CARVALHO, A.; GIUNCHETTI, R.C.; CARNEIRO, C.M.; MAYRINK, W.; TAFURI, W. L.; CORRÊA-OLIVEIRA, R. Systemic and compartmentalized immune response in canine visceral leishmaniasis. Veterinary immunology and immunopathology, v. 128, n. 1-3, p. 87-95, 2009.

ROGERS, K.A.; DEKERY, G.K.; MBOW, M.L.; GILLESPIE, R.D.; BRODSKYN, C.I.; TITUS, R.G. Type 1 and type 2 responses to Leishmania major. FEMS Microbiology Letters, v. 209, n. 1, p. 1-7, 2002.

SOARES, M.R.; DE MENDONÇA, I.L.; DO BONFIM, J.M.; RODRIGUES, J.A.; WERNECK, G.L.; COSTA, CH. Canine visceral leishmaniasis in Teresina, Brazil: Relationship between clinical features and infectivity for sand flies. Acta Tropica, v. 117, n. 1, p. 6-9, 2011.

SOLANO-GALLEGO, L.; MIRÓ, G.;KOUTINAS, A.; CARDOSO, L.; PENNISI, M.G.; FERRER, L.; BOURDEAU, P.; OLIVA, G.; BANETH, G. LeishVet guidelines for the practical management of canine leishmaniosis. Parasite and Vectors, v. 4, p. 86, 2011.

STERN, J.J.; OCA, M.J.; RUBIN, B.Y.; ANDERSON, S.L.; MURRAY, H.W. Role of L3T4+ and LyT-2+ cells in experimental visceral leishmaniasis. Journal of Immunology, v. 140, n. 11, p. 3971-7, 1988.

STRAUSS-AYALI, D.; BANETH, G.; JAFFE, C.L. Splenic immune responses during canine visceral leishmaniasis. Veterinary Research, v. 38, n. 4, p. 547-64, 2007.

TAFURI, W.L.; DE OLIVEIRA, M.R.; MELO, M.N. Canine visceral leishmaniosis: a remarkable histopathological picture of one case reported from Brazil. Veterinary Parasitology, v. 96, n. 3, p. 203-12, 2001.

THUNG, S.N.; GERBER, F. Liver biopsy. MAC SWENN, R.N.M. et al. In: Pathology of the liver. 3th ed. London: Churchill Livingstone, 1994. p.787-796.


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