Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
Resumo
Leishmaniose visceral canina (LVC) é uma zoonose causada por Leishmania (Leishmania) infantum chagasi no continente Americano. Poucos estudos demonstraram as características imunopatológicas em linfonodo poplíteo e fígado de cães infectados em área endêmica de Teresina, Piauí, Brasil. Assim, nós avaliamos as alterações histopatológicas e a presença de células T CD4+ e CD8+ em linfonodo poplíteo e fígado de nove cães com manifestações clínicas (G1) e sete cães sem manifestações clínicas (G2) naturalmente infectados com L. (L.) infantum chagasi. Fragmentos de linfonodo poplíteo e fígado foram obtidos para análise histopatológica e imunoistoquímica. As principais manifestações clínicas foram linfadenopatia (80%) e lesões de pele (70%). Na análise histopatológica do linfonodo foi observada uma depleção de células da região cortical. Não havia diferença significante entre os grupos entre células T CD4+ e CD8+ mas havia uma correlação positiva entre essas células em G1 e G2. No fígado, o infiltrado inflamatório era focal consistindo de células mononucleares nas regiões periportal e intralobular, em ambos os grupos. O infiltrado inflamatório era positivo na imunomarcação para células T CD4e CD8, mas sem diferença significante entre os grupos. Entretanto, havia uma correlação positiva entre as células T CD4+ e CD8+ no G1. Na análise da imunomarcação também mostrou mais amastigotas e antígenos de Leishmania no fígado e linfonodo poplíteo em G1 em relação ao G2. O papel das células T CD4+ e CD8+ parece não estar relacionada ao estado clínico dos cães com LVC.
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Referências
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